NOCTUA STIMULATED RAMAN IMAGING SYSTEM
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- Model: NOCTUA STIMULATED RAMAN IMAGING SYSTEM
Refined Laser Systems NOCTUA STIMULATED RAMAN IMAGING SYSTEM
Overview
- Add-on to Evident Scientific (form. Olympus) BX63, FV3000 or Nikon Ti2
- Fully-computer controlled & adjustment-freee
- Intrinsically confocal for imaging 3D specimens
- Minimum phototoxicity by near-infrared laser
LABEL-FREE IMAGING WITH HIGHEST CONTRAST 
NOCTUA stimulated Raman system offers highly specific imaging of living cells without fluorescent labels. Image contrast is generated by exciting intrinsic molecular bonds in the specimen, opening new windows into cellular processes such as lipid meta- bolism or pharmacokinetics. Observe cellular dynamics in real-time or long-term without perturbations of photobleaching or labeling. The image shows MCF7 cells acquired at 2850 cm-1 and 2950 cm-1 to highlight nuclei and cytoplasma.
IDENTIFY NUCLEI & CYTOPLASM WITHOUT STAINING
Noctua microscopy system allows imaging fresh and frozen tissue sections for pathological assessment without complex staining. The SRS image shows cell nuclei (purple) and cell bodies (pink) in mouse liver tissue. This label-free, virtual H&E contrast correlates well with classic H&E Staining.
REVEAL & EXPLORE THE HIDDEN STRUCTURES
Noctua microscopy system allows imaging structures and events in physiological relevant conditions, that are inaccessible with fluorescent labels. The SRS images show unsaturated fatty acids and cholesterol in C.elegans (upper panel). The lower panel shows dermal structures, formed by lipids, in the skin of a mouse ear (left) and a topically applied, unlabeled drug penetrating through the skin (right).
NOCTUA SPECIFICATIONS
| Microscope specifications (typical) |
||
|---|---|---|
| Field of view | 350 μm x 350 μm | |
| Excitation objective | 25x, NA 1.1 | |
| Collection condenser | NA 0.9 | |
| Resolution | < 500 nm | |
| Pixel dwell time | > 1 μs | |
| Laser specifications | Output A | Output B |
|---|---|---|
| Tuning range | 750-980 nm | 1025 - 1052 nm |
| Tuning speed | <100 ms | <100 ms |
| Average power | 100 - 250 mW | > 300 mW |
| Pulse duration | 7 - 10 ps | 2 - 3 ps |
| Covered wavenumbers | 700 - 3100 cm-1 | |
| Spectral bandwidth | < 12 cm -1 | |
| Repetition rate | 40 MHz | |
| Mechanicel dimensions (excluding microscope stativ) |
||
|---|---|---|
| Table top laser + scanner | 42 x 65 x 25 cm3 | |
| Supply unit (off table) | 43 x 45 x 26 cm3 | |
Refined Lasaer Systemwebsite
| File Name | Size | Link |
|---|---|---|
 Terms and Conditions V6
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207.42KiB | Download |
